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Parvalbumin

貨號 貨期 規格 / 價格 詢價

Parvalbumin

品牌

Swant

貨號

rPV100

產品分類

Recombinant Protein

研究領域

Parvalbumin

產品概述

Reconstitute with 10 or 100 ul bidistilled water. Freeze in small aliquots (e.g. 1 or 10 ug) and keep at -80°C. Avoid repeated freezing and thawing.

形式

This recombinant protein has been purified by FPLC. This protein can be used for immunoblots, absorption experiments in immunohistochemistry, radioimmunoassay and intracellular injection. For fluid-phase adsorption in immunohistochemistry we suggest the following procedure: - Dilute 1 ul of the mon oclonal antibody 235 (Swant) against parvalbumin in 5 ml of the usual buffer for immunohistochemistry (final dilution 1:5'000). - Add 1 ug of the recombinant protein to 1 ml of the diluted antibody solution and mix well. - Incubate for at least 6 hours in the cold. - Apply to tissue-sections and incubate for 3 days. - Complete the immunohistochemical reaction as usual (biotinylated second antibody, ABC-complex, DAB). As a result, the immunostaining should be strongly reduced or even completely prevented.

濃度

Rat parvalbumin (recombinant) produced in Escherichia Coli.

純度

Recombinant rat

試劑準備

lyophilized (10 or 100 ug in 100 ul of: 10 mM Tris-base, 70 mM NaCl, 1 mM EDTA, 1mM 脽-mercaptoethanol, pH 7.4).

產品描述

Purification: HPLC Lot No: 3.'94

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數量

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